PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total Cortactin protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.419~423 (P-I-Y-E-D) derived from Human CORTACTIN.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin(Ab-421) Antibody #AB21263(left) or the same antibody preincubated with blocking peptide(right).
Background
Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
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