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Raf1(Phospho-Ser259) AntibodyAB11006

  Catalog No. Package Size Price
AB11006-1 50ul $189.00
AB11006-2 100ul $279.00
AB11006-4 25ul $119.00

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Description
  • Product Name Raf1(Phospho-Ser259) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.
  • Target Name Raf1
  • Modification Phospho-Ser259
  • Alternative Names C-RAF; C-Raf; CRAF
  • Accession No. Swiss-Prot: P04049
    NCBI Protein: NP _002871.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • Raf1(Phospho-Ser259) Antibody - Absci

    Western blot analysis of extracts from 293 and MDA cells untreated or treated with PMA using Raf1(Phospho-Ser259) Antibody #AB11006.

  • Raf1(Phospho-Ser259) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody #AB11006.

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.

    Please let us know if you have published research using #AB11006 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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