Raf1(Phospho-Ser259) Antibody

Description

Product Name Raf1(Phospho-Ser259) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.
Target Name Raf1
Modification Phospho-Ser259
Alternative Names C-RAF; C-Raf; CRAF
Accession No. Swiss-Prot: P04049
NCBI Protein: NP _002871.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from 293 and MDA cells untreated or treated with PMA using Raf1(Phospho-Ser259) Antibody #AB11006.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody #AB11006.

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.

Published Papers

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