BRCA1(Ab-1423) Antibody

Description

Product Name BRCA1(Ab-1423) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous level of total BRCA1 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.1421~1425 (H-G-S-Q-P) derived from Human BRCA1.
Target Name BRCA1
Alternative Names RNF53
Accession No. Swiss-Prot: P38398
NCBI Protein: NP_009225.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 220kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from Hela and HT29 cells using BRCA1(Ab-1423) Antibody #AB21234.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1(Ab-1423) Antibody #AB21234(left) or the same antibody preincubated with blocking peptide(right).

Background

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Kim ST, et al. (2002) Genes Dev; 16(5): 560-570
Arlt MF, et al. (2004) Mol Cell Biol; 24(15): 6701-6709
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Greenberg RA, et al. (2006) Genes Dev; 20(1): 34-46
Tibbetts RS, et al. (2000) Genes Dev; 14(23): 2989-3002

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