eIF4E(Ab-209) Antibody

Description

Product Name eIF4E(Ab-209) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total elF4E protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.
Target Name eIF4E
Alternative Names mRNA cap-binding protein; eIF-4F 25 kDa subunit;
Accession No. Swiss-Prot: P06730
NCBI Protein: NP_001124150.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 25kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from Hela, HepG2 and 293 cells using eIF4E(Ab-209) Antibody #AB21226.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Ab-209) Antibody #AB21226(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using eIF4E(Ab-209) Antibody #AB21226.

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Li BD, et al. (1998) Ann Surg; 227(5): 756-763
Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520
De Gregorio E, et al. (2001) RNA; 7(1): 106-113
Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Ohlmann T, et al. (1996) EMBO J; 15(6): 1371-1382

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