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PHB(Phospho-Thr258) AntibodyAB11588

Catalog No.	Package Size	Price
AB11588-1	50ul	$189.00
AB11588-2	100ul	$279.00
AB11588-4	25ul	$119.00

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Description

Product Name PHB(Phospho-Thr258) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of PHB only when phosphorylated at threonine 258.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from Human PHB.
Target Name PHB
Modification Phospho-Thr258
Alternative Names Prohibitin; PHB1
Accession No. Swiss-Prot: P35232;
NCBI Gene ID: 5245;
NCBI Protein: NP_001268425.1.
SDS-PAGE MW 29
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from MCF, PC12, 293 and 3T3 cells using PHB (Phospho-Thr258) Antibody #AB11588.

Background

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Published Papers

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