CRMP-2 (Phospho-Ser522) Antibody

Description

Product Name CRMP-2 (Phospho-Ser522) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of DPYSL2 only when phosphorylated at serine 522.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Serine 522(K-T-S(p)-P-A) derived from Human CRMP-2.
Target Name CRMP-2
Modification Phospho-Ser522
Alternative Names CRMP2; DPYL2; DRP2; ULIP-2;
Accession No. Swiss-Prot#: Q16555;
NCBI Gene#: 1808;
NCBI Protein#: NP_001377.1.
SDS-PAGE MW 62kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HuvEc cells (Lane 2), using CRMP-2 (Phospho-Ser522) Antibody #AB11841. The lane on the left is treated with antigen-specific peptide.

Background

CRMP-2 is an enzyme with dihydropyrimidinase activity. Plays a role in RhoA-dependent signaling, through interaction with and regulation of Rho kinase. Plays a role in neurogenesis. Aberrantly expressed in fetal Down syndrome brain.

Goshima Y., Nature 376:509-514(1995).
Hamajima N., Gene 180:157-163(1996).
Kitamura K., DNA Res. 6:291-297(1999).

Published Papers

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