EPHA4 (Phospho-Tyr596) Antibody

Description

Product Name EPHA4 (Phospho-Tyr596) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 596 (R-T-Y(p)-V-D) derived from Human EPHA4 .
Target Name EPHA4
Modification Phospho-Tyr596
Alternative Names EK8; hEK8; EPH-like kinase 8;
Accession No. Swiss-Prot#: P54764;
NCBI Gene#: 2043;
NCBI Protein#: NP_004429.1.
SDS-PAGE MW 110kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody #AB11834. The lane on the left is treated with antigen-specific peptide.

Background

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity.

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Fu W.Y., Nat. Neurosci. 10:67-76(2007) [PubMed: 17143272].

Published Papers

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