EPHA2/5 (Phospho-Tyr594) Antibody

Description

Product Name EPHA2/5 (Phospho-Tyr594) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms
Specificity The Antibody detects endogenous levels of EPHA2 only when phosphorylated at tyrosine 594.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 594 (H-T-Y(p)-E-D) derived from Human EPHA2/5.
Target Name EPHA2/5
Modification Phospho-Tyr594
Alternative Names ARCC2; ECK; EPHA2;
Accession No. Swiss-Prot#: P29317/P54756;
NCBI Gene#: 1969/2044;
NCBI Protein#: NP_004422.2.
SDS-PAGE MW 110kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from JK cells (Lane 2), using EPHA2/5 (Phospho-Tyr594) Antibody #AB11833. The lane on the left is treated with antigen-specific peptide.

Background

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).
Gregory S.G., Nature 441:315-321(2006).

Published Papers

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