VAV3 (Phospho-Tyr173) Antibody

Description

Product Name VAV3 (Phospho-Tyr173) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of VAV3 only when phosphorylated at tyrosine 173.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 173 (E-V-Y(p)-E-D) derived from Human VAV3.
Target Name VAV3
Modification Phospho-Tyr173
Alternative Names VAV3; FLJ40431; Guanine nucleotide exchange factor VAV3;
Accession No. Swiss-Prot#: Q9UKW4;
NCBI Gene#: 10451;
NCBI Protein#: NP_006104.4.
SDS-PAGE MW 100kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from JK cells using VAV3 (Phospho-Tyr173) Antibody #AB11830.The lane on the right is treated with the antigen-specific peptide.

Background

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Trenkle T., Nucleic Acids Res. 26:3883-3891(1998).
Movilla N., Mol. Cell. Biol. 19:7870-7885(1999).

Published Papers

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