TOB1 (Phospho-Ser164) Antibody

Description

Product Name TOB1 (Phospho-Ser164) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1.
Target Name TOB1
Modification Phospho-Ser164
Alternative Names TOB; Transducer of erbB-2 1;
Accession No. Swiss-Prot#: P50616;
NCBI Gene#: 10140;
NCBI Protein#: NP_001230806.1.
SDS-PAGE MW 40kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody #AB11816. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody #AB11816 (left)or the same antibody preincubated with blocking peptide (right).

Background

This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

Matsuda S., Oncogene 12:705-713(1996).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

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