PLD2 (Phospho-Tyr169) Antibody

Description

Product Name PLD2 (Phospho-Tyr169) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2.
Target Name PLD2
Modification Phospho-Tyr169
Alternative Names PLD 2; PLD1C; choline phosphatase 2;
Accession No. Swiss-Prot#: O14939;
NCBI Gene#: 5338;
NCBI Protein#: NP_002654.3.
SDS-PAGE MW 95kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody #AB11813.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody #AB11813 (left)or the same antibody preincubated with blocking peptide (right).

Background

Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.

Steed P.M., FASEB J. 12:1309-1317(1998).
Lopez I., J. Biol. Chem. 273:12846-12852(1998).
Divecha N., EMBO J. 19:5440-5449(2000).

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