Neuro D (Phospho-Ser274) Antibody

Description

Product Name Neuro D (Phospho-Ser274) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of Neuro D only when phosphorylated at serine 274.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Serine 274(P-L-S(p)-P-P) derived from Human Neuro D.
Target Name Neuro D
Modification Phospho-Ser274
Alternative Names NDF1; NEUROD; NeuroD1; beta2;
Accession No. Swiss-Prot#: Q13562;
NCBI Gene#: 4760;
NCBI Protein#: NP_002491.2.
SDS-PAGE MW 36kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HeLa cells treated with UV using Neuro D (Phospho-Ser274) Antibody #AB11809.The lane on the right is treated with the antigen-specific peptide.

Background

NeuroD Acts as a differentiation factor during neurogenesis. Transcriptional activator. Binds to the insulin gene E-box.

Tamimi R., Genomics 34:418-421(1996).
Yokoyama M., Brain Res. Mol. Brain Res. 42:135-139(1996).
Miyachi T., Brain Res. Mol. Brain Res. 69:223-231(1999).

Published Papers

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