EPS15 (Phospho-Tyr849) Antibody

Description

Product Name EPS15 (Phospho-Tyr849) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of EPS15 only when phosphorylated at tyrosine 849.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15.
Target Name EPS15
Modification Phospho-Tyr849
Alternative Names AF1P; MLLT5; EPS15; Protein Eps15;
Accession No. Swiss-Prot#: P42566;
NCBI Gene#: 2060;
NCBI Protein#: NP_001972.1.
SDS-PAGE MW 100kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody #AB11800. The lane on the left is treated with antigen-specific peptide.

Background

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Wong W.T., Oncogene 9:1591-1597(1994).
Bernard O.A., Oncogene 9:1039-1045(1994).

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