Claudin 7 (Phospho-Tyr210) Antibody

Description

Product Name Claudin 7 (Phospho-Tyr210) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of Claudin 7 only when phosphorylated at tyrosine 210.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 210 (S-K-E-Y(p)-V) derived from Human Claudin 7.
Target Name Claudin 7
Modification Phospho-Tyr210
Alternative Names CEPTRL2; claudin 7; CLD7; CPETRL2; CLDN7
Accession No. Swiss-Prot#: O95471;
NCBI Gene#: 1366;
NCBI Protein#: NP_001171951.1.
SDS-PAGE MW 32kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from 3T3 cells (Lane 2), using Claudin 7 (Phospho-Tyr210) Antibody #AB11794. The lane on the left is treated with antigen-specific peptide.

Background

Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells.

Keen T.J.; Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

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