CAD (Phospho-Thr456) Antibody

Description

Product Name CAD (Phospho-Thr456) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of CAD only when phosphorylated at threonine 456.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD.
Target Name CAD
Modification Phospho-Thr456
Alternative Names PYR1; CAD protein; EC 2.1.3.2; EC 3.5.2.3;
Accession No. Swiss-Prot#: P27708;
NCBI Gene#: 790;
NCBI Protein#: NP_004332.2.
SDS-PAGE MW 242kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Immunohistochemistry: 1:50~1:100

Images

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody #AB11789 (left)or the same antibody preincubated with blocking peptide (right).

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255(1996).
Davidson J.N., DNA Cell Biol. 9:667-676(1990).
Olsen J.V., Cell 127:635-648(2006).

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