B-Raf (Phospho-Thr753) Antibody

Description

Product Name B-Raf (Phospho-Thr753) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 753 (P-K-T(p)-P-L) derived from Human B-Raf.
Target Name B-Raf
Modification Phospho-Thr753
Alternative Names B-RAF; RAFB1; RMIL; p94;
Accession No. Swiss-Prot#: P15056;
NCBI Gene#: 673;
NCBI Protein#: NP_004324.2.
SDS-PAGE MW 84kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody #AB11786.The lane on the right is treated with the antigen-specific peptide.

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W., Science 300:767-772(2003)

Published Papers

let us know

Customer Reviews and Rankings

No comment