AIRE (Phospho-Ser156) Antibody

Description

Product Name AIRE (Phospho-Ser156) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of AIRE only when phosphorylated at serine 156.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Serine156 P-G-S(p)-Q-L) derived from Human AIRE.
Target Name AIRE
Modification Phospho-Ser156
Alternative Names AIRE1; APECED; APS1; APSI; PGA1
Accession No. Swiss-Prot#: O43918;
NCBI Gene#: 326;
NCBI Protein#: NP_000374.1.
SDS-PAGE MW 50kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HeLa cells treated with Hu using AIRE (Phospho-Ser156) Antibody #AB11782.The lane on the right is treated with the antigen-specific peptide.

Background

The function of the protein encoded by this gene is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE-1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE-2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE-3 includes the same exon 1-8-9 sequences as found in AIRE-2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized.

Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).

Published Papers

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