c-Jun(Phospho-Ser73) Antibody

Description

Product Name c-Jun(Phospho-Ser73) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from Human c-Jun.
Target Name c-Jun
Modification Phospho-Ser73
Alternative Names AH119; AP1; Jun A; c-Jun; p39
Accession No. Swiss-Prot: P05412
NCBI Protein: NP_002219.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody #AB11003.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody #AB11003(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody #AB11003.