WWOX (Phospho-Tyr33) Antibody

Description

Product Name WWOX (Phospho-Tyr33) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.
Target Name WWOX
Modification Phospho-Tyr33
Alternative Names FOR; WOX1; Aberrant WW domain-containing oxidoreductase;
Accession No. Swiss-Prot#: Q96KM3;
NCBI Gene#: 51741;
NCBI Protein#: NP_057457.1.
SDS-PAGE MW 55kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody #AB11779.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody #AB11779 (left)or the same antibody preincubated with blocking peptide (right).

Background

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.

Bednarek A.K., Cancer Res. 60:2140-2145(2000).
Ried K., Hum. Mol. Genet. 9:1651-1663(2000).
Bednarek A.K., Cancer Res. 61:8068-8073(2001).

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