Androgen Receptor (Phospho-Tyr363) Antibody

Description

Product Name Androgen Receptor (Phospho-Tyr363) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms
Specificity The antibody detects endogenous levels of Androgen Receptor only when phosphorylated at tyrosine 363.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 363 (D-Y-Y(p)-N-F) derived from Human Androgen Receptor.
Target Name Androgen Receptor
Modification Phospho-Tyr363
Alternative Names ANDR; Androgen receptor; Dihydrotestosterone receptor; NR3C4;
Accession No. Swiss-Prot#: P10275;
NCBI Gene#: 367;
NCBI Protein#: NP_000035.2.
SDS-PAGE MW 85kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HuvEc cells (Lane 2), COS7 cells (Lane 3) and JK cells (Lane 4), using Androgen Receptor (Phospho-Tyr363) Antibody #AB11761. The lane on the left is treated with antigen-specific peptide.

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Lubahn D.B., Mol. Endocrinol. 2:1265-1275(1988).
Chang C., Proc. Natl. Acad. Sci. U.S.A. 85:7211-7215(1988).
Tilley W.D., Proc. Natl. Acad. Sci. U.S.A. 86:327-331(1989).

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