APP(Ab-668) Antibody

Description

Product Name APP(Ab-668) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total APP protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.666~670 (A-V-T-P-E ) derived from Human APP.
Target Name APP
Alternative Names AAA; AD1; PN2; ABPP; APPI
Accession No. Swiss-Prot: P05067
NCBI Protein: NP_000475.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 100-140 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from mouse brain tissue using APP(Ab-668) Antibody #AB21204 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed MCF cells using APP(Ab-668) Antibody #AB21204.

Background

APP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122
Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091

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