OSR1 (Phospho-Thr185) Antibody

Description

Product Name OSR1 (Phospho-Thr185) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of OSR1 only when phosphorylated at threonine 185.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine185(R-K-T(p)-F-V) derived from Human OSR1.
Target Name OSR1
Modification Phospho-Thr185
Alternative Names OXSR1; kinase OSR1; Oxidative-stress responsive 1;
Accession No. Swiss-Prot#: O95747;
NCBI Gene#: 9943;
NCBI Protein#: NP_005100.1.
SDS-PAGE MW 65kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HepG2 cells treated with serum using OSR1 (Phospho-Thr185) Antibody #AB11746.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using OSR1 (Phospho-Thr185) antibody #AB11746 (left)or the same antibody preincubated with blocking peptide (right).

Background

Oxidative-stress responsive 1 gene is located in the vicinity of three others genes - GOLGA4, ITGA9 and HYA22 on chromosome 3. These four genes are considered to be candidate tumor suppressors. Oxidative-stress responsive 1 protein has similarity to human Ste20/oxidant stress response kinase-1 and is thought to be involved in the response to oxidative stress

Tamari M., J. Hum. Genet. 44:116-120(1999).
Kikuno R., DNA Res. 6-197-205(1999).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

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