MAP2K7 (Phospho-Thr275) Antibody

Description

Product Name MAP2K7 (Phospho-Thr275) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of MAP2K7 only when phosphorylated at threonine 275.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 275(A-K-T(p)-R-S) derived from Human MAP2K7.
Target Name MAP2K7
Modification Phospho-Thr275
Alternative Names PRKMK7; JNKK2; MKK7; MAP2K7; MP2K7
Accession No. Swiss-Prot#: O14733;
NCBI Gene#: 5609;
NCBI Protein#: NP_660186.1.
SDS-PAGE MW 43kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody #AB11743. The lane on the left is treated with antigen-specific peptide.

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.

Wu Z.,Mol. Cell. Biol. 17:7407-7416(1997).
Lu X.,J. Biol. Chem. 272:24751-24754(1997).
The MGC Project Team;Genome Res. 14:2121-2127(2004).

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