MAP2K3 (Phospho-Thr222) Antibody

Description

Product Name MAP2K3 (Phospho-Thr222) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 222(A-K-T(p)-M-D) derived from Human MAP2K3 .
Target Name MAP2K3
Modification Phospho-Thr222
Alternative Names MAP2K3; MAPKK 3; MEK3; MP2K3; PRKMK3
Accession No. Swiss-Prot#: P46734;
NCBI Gene#: 5606;
NCBI Protein#: NP_659731.1.
SDS-PAGE MW 39kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody #AB11741.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody #AB11741 (left)or the same antibody preincubated with blocking peptide (right).

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

Derijard B., Science 267:682-685(1995).
Moriguchi T., J. Biol. Chem. 271:26981-26988(1996).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

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