CK-1α (Phospho-Tyr294) Antibody

Description

Product Name CK-1α (Phospho-Tyr294) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.
Target Name CK-1α
Modification Phospho-Tyr294
Alternative Names KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I
Accession No. Swiss-Prot#: P48729/Q8N752;
NCBI Gene#: 1452/122011;
NCBI Protein#: NP_001883.4.
SDS-PAGE MW 37kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1α (Phospho-Tyr294) Antibody #AB11728.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1α (Phospho-Tyr294) antibody #AB11728 (left)or the same antibody preincubated with blocking peptide (right).

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Tapia C., FEBS Lett. 349:307-312(1994).
Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

Published Papers

let us know

Customer Reviews and Rankings

No comment