ABL1 (Phospho-Thr735) Antibody

Description

Product Name ABL1 (Phospho-Thr735) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine735 (S-V-T(p)-L-P) derived from Human ABL1.
Target Name ABL1
Modification Phospho-Thr735
Alternative Names Abelson murine leukemia viral oncogene 1; ABL1; c-ABL; JTK7; p150
Accession No. Swiss-Prot#: P00519;
NCBI Gene#: 25;
NCBI Protein#: NP_005148.2.
SDS-PAGE MW 135kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from COS cells treated with EGF using ABL1 (Phospho-Thr735) Antibody #AB11725.The lane on the right is treated with the antigen-specific peptide.

Background

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

Fainstein E., Oncogene 4:1477-1481(1989).
Shtivelman E., Cell 47:277-284(1986).
Chissoe S.L., Genomics 27:67-82(1995).

Published Papers

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