PDK1 (Phospho-Tyr9) Antibody

Description

Product Name PDK1 (Phospho-Tyr9) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of PDK1 only when phosphorylated at tyrosine 9.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 9(A-L-Y(p)-D-A) derived from Human PDK1.
Target Name PDK1
Modification Phospho-Tyr9
Alternative Names hPDK1; PDPK1; mPDK1; PkB kinase;
Accession No. Swiss-Prot#: O15530;
NCBI Gene#: 5170;
NCBI Protein#: NP_002604.1.
SDS-PAGE MW 63kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Immunohistochemistry: 1:50~1:100

Images

Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody #AB11719 (left)or the same antibody preincubated with blocking peptide (right).

Background

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Alessi D.R., Curr. Biol. 7:261-269(1997).
Alessi D.R.,Curr. Biol. 7:776-789(1997).
Stephens L.R.,Science 279:710-714(1998)

Published Papers

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