Rac1(Ab-71) Antibody

Description

Product Name Rac1(Ab-71) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total Rac1 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.69~73 (P-L-S-Y-P) derived from Human Rac1.
Target Name Rac1
Alternative Names Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; p21-Rac1
Accession No. Swiss-Prot: P63000
NCBI Protein: NP_008839.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 21kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from 293 and Hela cells using Rac1(Ab-71) Antibody #AB21201 .
Immunofluorescence staining of methanol-fixed Hela cells using Rac1(Ab-71) Antibody #AB21201.

Background

Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Aoki K, et al. (2005) Mol Biol Cell; 16(5): 2207

Published Papers

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