CD227/MUC1 (Phospho-Tyr1229) Antibody

Description

Product Name CD227/MUC1 (Phospho-Tyr1229) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu
Specificity The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1.
Target Name CD227/MUC1
Modification Phospho-Tyr1229
Alternative Names PUM; EMA; H23AG; MUC-1; PEM
Accession No. Swiss-Prot#: P15941;
NCBI Gene#: 4582;
NCBI Protein#: NP_001018016.1.
SDS-PAGE MW 170kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody #AB11707.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody #AB11707 (left)or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody #AB11707.

Background

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Lan M.S., J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)

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