TGF β Receptor II (Phospho-Ser225/250) Antibody

Description

Product Name TGF β Receptor II (Phospho-Ser225/250) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of TGF β Receptor II only when phosphorylated at serine 225/250.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Serine 225/250(D-R-S(p)-D-I) derived from Human TGF β Receptor II.
Target Name TGF β Receptor II
Modification Phospho-Ser225/250
Alternative Names TGF-betaR2; TGFR2; kinase TGF-beta-R2;
Accession No. Swiss-Prot#: P37173;
NCBI Gene#: 7048;
NCBI Protein#: NP_003233.4.
SDS-PAGE MW 64kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Immunohistochemistry: 1:50~1:100

Images

Immunohistochemical analysis of paraffin-embedded human brain tissue using TGF β Receptor II (Phospho-Ser225/250) antibody #AB11700 (left)or the same antibody preincubated with blocking peptide (right).

Background

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis.

RC Tripathi, Invest. Ophthalmol. Vis. Sci., Jan 1993; 34: 260.
N Chegini, Endocrinology, Jul 1994; 135: 439.
GW Mulheron, Endocrinology, Dec 1992; 131: 2609

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