CSFR (Phospho-Tyr561) Antibody

Description

Product Name CSFR (Phospho-Tyr561) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of CSFR only when phosphorylated at tyrosine 561.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 561 (N-S-Y(p)-T-F) derived from Human CSFR .
Target Name CSFR
Modification Phospho-Tyr561
Alternative Names CD115; CSF-1-R; CSFMR; FMS; M-CSFR
Accession No. Swiss-Prot#: P07333;
NCBI Gene#: 1436;
NCBI Protein#: NP_001275634.1.
SDS-PAGE MW 130kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HepG2 cells treated with PMA using CSFR (Phospho-Tyr561) Antibody #AB11688.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain, using CSFR (Phospho-Tyr561) antibody #AB11688 (left)or the same antibody preincubated with blocking peptide (right).

Background

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development.

Hampe A., Oncogene Res. 4:9-17(1989).
Andre C., Genomics 39:216-226(1997).
Liu T., J. Proteome Res. 4:2070-2080(2005).

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