C/EBP-ε (Phospho-Thr74) Antibody

Description

Product Name C/EBP-ε (Phospho-Thr74) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 174 (L-A-T(p)-A-A) derived from Human C/EBP-ε.
Target Name C/EBP-ε
Modification Phospho-Thr74
Alternative Names CEBPE; C/EBP epsilon; CCAAT/enhancer binding protein epsilon;
Accession No. Swiss-Prot#: Q15744;
NCBI Gene#: 1053;
NCBI Protein#: NP_001796.2.
SDS-PAGE MW 34kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HUVEC cells treated with UV using C/EBP-ε (Phospho-Thr74) Antibody #AB11686.The lane on the right is treated with the antigen-specific peptide.

Background

C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.
Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.
Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.
Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

Published Papers

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