Bloom Syndrome (Phospho-Thr99) Antibody

Description

Product Name Bloom Syndrome (Phospho-Thr99) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.
Target Name Bloom Syndrome
Modification Phospho-Thr99
Alternative Names RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
Accession No. Swiss-Prot#: P54132;
NCBI Gene#: 641;
NCBI Protein#: NP_000048.1.
SDS-PAGE MW 159kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody #AB11681.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody #AB11681 (left)or the same antibody preincubated with blocking peptide (right).

Background

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).

Ellis N.A., Cell 83:655-666(1995).
Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
Pichierri P., EMBO J. 23:3154-3163(2004).

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