MSK1(Ab-376) Antibody

Description

Product Name MSK1(Ab-376) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of total MSK1 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.374~378 (G-Y-S-F-V) derived from Human MSK1.
Target Name MSK1
Alternative Names MSPK1; RPS6KA5;
Accession No. Swiss-Prot: O75582
NCBI Protein: NP_004746.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 90kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HUVEC cells using MSK1(Ab-376) Antibody #AB21198 and the same antibody preincubated with blocking peptide.

Background

Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

Roux PP, et al. (2004) Microbiol Mol Biol Rev. Jun; 68(2): 320-344
McCOY C, et al. (2005) Biochem J. Apr 15; 387(Pt 2): 507-517

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