GSK3β(Phospho-Ser9) Antibody

Description

Product Name GSK3β(Phospho-Ser9) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.
Target Name GSK3β
Modification Phospho-Ser9
Alternative Names Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta
Accession No. Swiss-Prot: P49841
NCBI Protein: NP_001139628.1
SDS-PAGE MW 46kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3β(Phospho-Ser9) Antibody #AB11002.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #AB11002 (left) or the same antibody preincubated with blocking peptide #BP51002 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody #AB11002.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #AB11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #AB11002.
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #AB11002.

Background

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.

Published Papers

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