CD3 ζ (Phospho-Tyr142) Antibody

Description

Product Name CD3 ζ (Phospho-Tyr142) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms
Specificity The antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 142 (G-L-Y(p)-Q-G) derived from Human CD3 ζ.
Target Name CD3 ζ
Modification Phospho-Tyr142
Alternative Names T3Z; CD3-zeta; TCRZ; T-cell receptor T3 zeta chain;
Accession No. Swiss-Prot#: P20963;
NCBI Gene#: 919;
NCBI Protein#: NP_000725.1.
SDS-PAGE MW 20kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 ζ (Phospho-Tyr142) Antibody #AB11655. The lane on the left is treated with antigen-specific peptide.

Background

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029.
Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423.
Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330.
Hisashi Arase, J. Immunol., Jan 2001; 166: 21.

Published Papers

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