Arrestin 1 (Phospho-Ser412) Antibody

Description

Product Name Arrestin 1 (Phospho-Ser412) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1.
Target Name Arrestin 1
Modification Phospho-Ser412
Alternative Names ARR1; arrestin 2; arrestin beta 1; beta-arrestin-1;
Accession No. Swiss-Prot#: P49407;
NCBI Gene#: 408;
NCBI Protein#: NP_004032.2.
SDS-PAGE MW 50kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody #AB11654.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody #AB11654 (left)or the same antibody preincubated with blocking peptide (right).

Background

Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.

Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430.
D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375.
Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305.
Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.

Published Papers

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