p130 Cas (Phospho-Tyr410) Antibody

Description

Product Name p130 Cas (Phospho-Tyr410) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 410.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 410(G-V-Y(p)-A-V) derived from Human p130 Cas .
Target Name p130 Cas
Modification Phospho-Tyr410
Alternative Names BCAR1; CRKAS; CRK-ASSOCIATED SUBSTRATE;
Accession No. Swiss-Prot#: P56945;
NCBI Gene#: 9564;
NCBI Protein#: NP_055382.2.
SDS-PAGE MW 130kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody #AB11651. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody #AB11651 (left)or the same antibody preincubated with blocking peptide (right).

Background

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

Katarzyna Modzelewska, J. Biol. Chem., Dec 2006; 281: 37527 - 37535.
Kevin Ogden, Am J Physiol Heart Circ Physiol, Dec 2006; 291: H2857 - H2863.
Sangkil Nam, Cancer Res., Oct 2005; 65: 9185 - 9189.
Norikazu Yamana, Mol. Cell. Biol., Sep 2006; 26: 6844 - 6

Published Papers

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