Caspase 9 (Phospho-Thr125) Antibody

Description

Product Name Caspase 9 (Phospho-Thr125) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.
Target Name Caspase 9
Modification Phospho-Thr125
Alternative Names APAF-3; MCH6; RNCASP9; CASP-9; ICE-LAP6
Accession No. Swiss-Prot#: P55211;
NCBI Gene#: 842;
NCBI Protein#: NP_001220.2.
SDS-PAGE MW 47kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody #AB11649.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody #AB11649 (left)or the same antibody preincubated with blocking peptide (right).

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Duan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999)

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