Synaptotagmin (Phospho-Thr202) Antibody

Description

Product Name Synaptotagmin (Phospho-Thr202) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 202(R-K-T(p)-L-N) derived from Human Synaptotagmin.
Target Name Synaptotagmin
Modification Phospho-Thr202
Alternative Names LAG; LAP18; STN1; Prosolin; stathmin
Accession No. Swiss-Prot#: P21579;
NCBI Gene#: 6857;
NCBI Protein#: NP_001129277.1
SDS-PAGE MW 60kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody #AB11645. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody #AB11645 (left)or the same antibody preincubated with blocking peptide (right).

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.

E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.
Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143.
Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909.
Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

Published Papers

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