IGFBP-3 (Phospho-Ser183) Antibody

Description

Product Name IGFBP-3 (Phospho-Ser183) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Rt
Specificity The antibody detects endogenous level of IGFBP-3 only when phosphorylated at serine 183.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 183(K-D-S(p)-Q-R) derived from Human IGFBP-3 .
Target Name IGFBP-3
Modification Phospho-Ser183
Alternative Names IBP-3; IBP3; IGF-binding protein 3; IGFBP3; Insulin-like growth factor binding protein 3 precursor
Accession No. Swiss-Prot#: P17936;
NCBI Gene#: 3486;
NCBI Protein#: NP_000589.2
SDS-PAGE MW 30kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody #AB11999.The lane on the right is treated with the antigen-specific peptide.

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Zhang Q, Steinle JJ (2013)Invest Ophthalmol Vis Sci 54, 3052-7 . Cobb LJ, Liu B, Lee KW, Cohen P (2006) Cancer Res 66, 10878-84 .

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