PECAM-1 (Phospho-Tyr713) Antibody

Description

Product Name PECAM-1 (Phospho-Tyr713) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of PECAM-1 only when phosphorylated at tyrosine 713.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Tyrosine 713(T-V-Y(p)-S-E) derived from Human PECAM-1.
Target Name PECAM-1
Modification Phospho-Tyr713
Alternative Names CD31; CD31 antigen; EndoCAM; PEC1; PECAM
Accession No. Swiss-Prot#: P16284;
NCBI Gene#: 5175;
NCBI Protein#: NP_000433.4
SDS-PAGE MW 82 150kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Jurkat tissue using PECAM-1 (Phospho-Tyr713) antibody #AB11995.The lane on the right is treated with the antigen-specific peptide.

Background

Induces susceptibility to atherosclerosis By similarity. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.

Garnacho C, et al. (2008) Blood 111, 3024-33. Udell CM, et al. (2006) J Biol Chem 281, 20949-57 . O'Brien CD, Cao G, Makrigiannakis A, DeLisser HM (2004)Am J Physiol Cell Physiol 287, C1103-13.

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