Cyclin D1 (Phospho-Ser90) Antibody

Description

Product Name Cyclin D1 (Phospho-Ser90) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of Cyclin D1 only when phosphorylated at serine 90.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 90(F-L-S(p)-L-E) derived from Human Cyclin D1 .
Target Name Cyclin D1
Modification Phospho-Ser90
Alternative Names BCL1; CCND1; CYL-1; PRAD1; PRAD1 oncogene
Accession No. Swiss-Prot#: P24385;
NCBI Gene#: 595;
NCBI Protein#: NP_444284.1
SDS-PAGE MW 31kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from K562 tissue using Cyclin D1 (Phospho-Ser90) antibody #AB11986.The lane on the right is treated with the antigen-specific peptide.

Background

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Sewing A, M¨¹ller R (1994) Oncogene 9, 2733-6

Published Papers

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