VEGFR1 (Phospho-Tyr1213) Antibody

Description

Product Name VEGFR1 (Phospho-Tyr1213) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of VEGFR1 only when phosphorylated at tyrosine 1213.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Tyrosine 1213(V-R-Y(p)-V-N) derived from Human VEGFR1.
Target Name VEGFR1
Modification Phospho-Tyr1213
Alternative Names FLT; FLT1; FRT; Flt-1; Fms-like tyrosine kinase 1
Accession No. Swiss-Prot#: P17948;
NCBI Gene#: 2321;
NCBI Protein#: NP_001153392.1
SDS-PAGE MW 90kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HeLa cells treated with UV using Phospho-VEGFR1 (Tyr1213) antibody #AB11985.The lane on the right is treated with the antigen-specific peptide.

Background

Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Raghu H, et al. (2012) Mol Oncol 6, 33-47
Ito N, Huang K, Claesson-Welsh L (2001) Cell Signal 13, 849-54
Ito N, Wernstedt C, Engstr?m U, Claesson-Welsh L (1998) J Biol Chem 273, 23410-8

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