p70 S6 Kinase (Phospho-Thr389/412) Antibody

Description

Product Name p70 S6 Kinase (Phospho-Thr389/412) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389/tyrosine 412.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine389/412(G-F-T(p)-Y-V) derived from Human p70 S6 Kinase .
Target Name p70 S6 Kinase
Modification Phospho-Thr389/412
Alternative Names KS6B1; P70-S6K; RPS6KB1; Ribosomal protein S6 kinase; polypeptide 1
Accession No. Swiss-Prot#: P23443;
NCBI Gene#: 6198;
NCBI Protein#: NP_001258971.1
SDS-PAGE MW 70kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) antibody #AB11974.The lane on the right is treated with the antigen-specific peptide.

Background

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Hong S, et al. (2014) J Biol Chem
Puustinen P, et al. (2014) CJ Cell Biol 204, 713-27
Martineau Y, et al. (2014) Mol Cell Biol 34, 1046-53

Published Papers

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