c-Met (Phospho-Tyr1003) Antibody

Description

Product Name c-Met (Phospho-Tyr1003) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of c-Met only when phosphorylated at tyrosine 1003.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 1003 (V-D-Y(p)-R-A) derived from Human c-Met.
Target Name c-Met
Modification Phospho-Tyr1003
Alternative Names HGF receptor; HGF-SF receptor; Hepatocyte growth factor receptor precursor; Hepatocyte growth factor receptor precursor; Met proto- oncogene tyros
Accession No. Swiss-Prot#: P08581;
NCBI Gene#: 4233;
NCBI Protein#: NP_000236.2
SDS-PAGE MW 155kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HepG2 tissue using c-Met (Phospho-Tyr1003) antibody #AB11971.The lane on the right is treated with the antigen-specific peptide.

Background

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Usatyuk PV, et al. (2014)J Biol Chem. Asiedu MK, et al. (2014) Oncogene 33, 1316-24. Dulak AM, et al. (2011) Oncogene 30, 3625-35.

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