BLNK (Phospho-Tyr96) Antibody

Description

Product Name BLNK (Phospho-Tyr96) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of BLNK only when phosphorylated at tyrosine 96.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 96(D-S-Y(p)-E-P) derived from Human BLNK .
Target Name BLNK
Modification Phospho-Tyr96
Alternative Names B-cell linker protein; LY57; SLP-65; SLP65;
Accession No. Swiss-Prot#: Q8WV28;
NCBI Gene#: 29760;
NCBI Protein#: NM_001114094.1
SDS-PAGE MW 50kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) antibody #AB11964.The lane on the right is treated with the antigen-specific peptide.

Background

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling.

Wang X, et al. (2012)J Biol Chem 287, 11037-48. Patterson HC, et al. (2006)Immunity 25, 55-65 . Chiu CW, et al. (2002)EMBO J 21, 6461-72.

Published Papers

let us know

Customer Reviews and Rankings

No comment