FAK (Phospho-Tyr576) Antibody

Description

Product Name FAK (Phospho-Tyr576) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Tyrosine576 (S-T-Y(p)-Y-K) derived from Human FAK.
Target Name FAK
Modification Phospho-Tyr576
Alternative Names FADK 1; FAK1; Focal adhesion kinase 1; PTK2; pp125FAK
Accession No. Swiss-Prot#: Q05397;
NCBI Gene#: 5747;
NCBI Protein#: NP_001186578.1
SDS-PAGE MW 125kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from NIH-3T3 tissue using FAK (Phospho-Tyr576) antibody #AB11957.The lane on the right is treated with the antigen-specific peptide.

Background

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Tong L, et al. (2013)Biochim Biophys Acta 1833, 1304-15. Chen S, et al. (2013)Sci Signal 6, ra40. Tian H, et al. (2012)EMBO J 31, 3885-900.

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