SREBP-1 (Phospho-Ser439) Antibody

Description

Product Name SREBP-1 (Phospho-Ser439) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of SREBP-1 only when phosphorylated at serine 439.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine439(Q-S-S(p)-P-L) derived from Human SREBP-1 .
Target Name SREBP-1
Modification Phospho-Ser439
Alternative Names SRBP1; SREBF1; SREBP1; sterol regulatory element-binding protein 1;
Accession No. Swiss-Prot#: P36956;
NCBI Gene#: 6720;
NCBI Protein#: NP_001005291.1
SDS-PAGE MW 122kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Jurkat cells treated with TNF using Phospho-SREBP-1 (Ser439) antibody #AB11951.The lane on the right is treated with the antigen-specific peptide.

Background

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway By similarity. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

Bengoechea-Alonso MT, Ericsson J (2006) Cell Cycle 5, 1708-18

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