ATPase (Phospho-Ser16) Antibody

Description

Product Name ATPase (Phospho-Ser16) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of ATPase only when phosphorylated at serine 16.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 16 (A-V-S(p)-E-Q) derived from Human ATPase.
Target Name ATPase
Modification Phospho-Ser16
Alternative Names A1A1; AT1A1; ATP1A1; K-ATPase 1; Sodium pump 1
Accession No. Swiss-Prot#: P05023;
NCBI Gene#: 476;
NCBI Protein#: NP_000692.2
SDS-PAGE MW 112kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from 293 cells treated with PMA using Phospho-ATPase (Ser16) antibody #AB11938.The lane on the right is treated with the antigen-specific peptide.

Background

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Kava L, Rossi NF, Mattingly R, Yingst DR (2012) Am J Hypertens 25, 487-91
Soltoff SP, Asara JM, Hedden L (2010)J Biol Chem 285, 36330-8
Efendiev R, et al. (2006) FEBS Lett 580, 5067-70

Published Papers

let us know

Customer Reviews and Rankings

No comment